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Article

Significant improvement of the thermostability and catalytic efficiency of β-glucosidase Bgl59 via cysteine engineering and rational design.

International journal of biological macromolecules - 1 Feb 2026

Li Lanlan, Liu Ying, Dong Panpan, Zhang Hong, Li Lin, Gao Min, Wu Hanyu, Sun Lifang, Zhang Su, You Lianghao, Fu Lei, Xiao Fangnan, Hu Xuefeng, Wu Yunkun

Abstract excerpt

β-glucosidase (BGL) is essential in high-temperature industrial processes, but naturally occurring enzymes often lack sufficient thermostability. Using the wild-type Bgl59 from Devosia psychrophilaas the template, we first constructed the double-cysteine mutant A153C-N213C via disulfide-bond design. Although no disulfide bond was detected, this mutant showed improved thermal stability, including a + 2.5 °C...

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PMID: 41638280
DOI: 10.1016/j.ijbiomac.2026.150691

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