Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Semirational Engineering of α-Galactosidase for Improvement of Its Thermostability and Transglycosylation Ability.

Journal of agricultural and food chemistry - 6 May 2026

Fan Yan, Zhang Zhichao, Li Xiaofeng, Li Ruopeng, Fan Puxi, Bai Weidong, Fei Yongtao, Zhao Wenhong, Lai Xueneng

Abstract excerpt

α-Galactosidase catalyzes the transglycosylation reaction for galactooligosaccharides (GOS) synthesis. However, its strong hydrolytic activity limits the yield of oligosaccharides. Here, semirational design was used to engineer α-galactosidase AglB from Lactobacillus amylolyticus L6, generating three mutants with improved transglycosylation. K480R and H203P exhibited different functional characteristics. The...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Oligosaccharides
Share to alpha-Galactosidase

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Biocatalysis

Share to Enzyme Stability

Share to Hot Temperature

Share to Hydrolysis

Share to Lactobacillus

Share to Protein Engineering

Source

Identifiers

PMID: 42018280
DOI: 10.1021/acs.jafc.6c01014

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 17 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Semirational Engineering of α-Galactosidase for Improvement of Its Thermostability and Transglycosylation Ability.PMID 42018280Read ↗

Enhancing β-Galactosidase Performance for Galactooligosaccharides Preparation via Strategic Glucose Re-Tunneling.Semantic proximity Read ↗

Gly343 mutation enhances gentiobiose conversion efficiency of GH3 family β-glucosidase from Thermotoga maritima STB25.Semantic proximity Read ↗

Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase.Semantic proximity Read ↗

Significant improvement of the thermostability and catalytic efficiency of β-glucosidase Bgl59 via cysteine engineering and rational design.Semantic proximity Read ↗

Engineering of β-glucosidase for rare ginsenoside biosynthesis via distal conformational fine-tuning and sequential degradation control.Semantic proximity Read ↗

Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope.Semantic proximity Read ↗

The two-step strategy for enhancing the specific activity and thermostability of alginate lyase AlyG2 with mechanism for improved thermostability.Semantic proximity Read ↗

Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase.Semantic proximity Read ↗

Structural features of Aspergillus niger β-galactosidase define its activity against glycoside linkages.Semantic proximity Read ↗

Enhancing the catalytic activity of a GH5 processive endoglucanase from Bacillus subtilis BS-5 by site-directed mutagenesis.Semantic proximity Read ↗

Glucansucrase Gtf180-ΔN of Lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules.Semantic proximity Read ↗

Efficiency enhancement in Aspergillus niger α-L-rhamnosidase reverse hydrolysis by using a tunnel site rational design strategy.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.