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Computational Methods to Engineer Cas Proteins for Efficient Genome Editing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Fatmi Muhammad Qaiser, Nadeem Aneeqa, Abbasov Mehraj, Sajjad Muhammad

Abstract excerpt

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) system has revolutionized genome editing through programmable, sequence-specific deoxyribonucleic acid (DNA) targeting. Yet, its broader application remains limited by off-target effects and context-dependent efficiency. To address these challenges, we present an integrated computational protocol with easy-to-do...

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PMID: 41174319
DOI: 10.1007/978-1-0716-4828-5_17

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