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Article

Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a.

Nucleic acids research - 25 Jan 2024

Sinha Souvik, Molina Vargas Adrian M, Arantes Pablo R, Patel Amun, O'Connell Mitchell R, Palermo Giulia

Abstract excerpt

Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which makes it a promising tool for RNA detection. It utilizes a CRISPR RNA (crRNA) to target RNA sequences and trigger a composite active site formed by two 'Higher Eukaryotes and Prokaryotes Nucleotide' (HEPN) domains, cleaving any solvent-exposed RNA. In this system, an intriguing form of allosteric communication controls the...

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Identifiers

PMID: 38033317
DOI: 10.1093/nar/gkad1127
PMCID: PMC10810222

Genes

HEPN1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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