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Efficient glycosylase-mediated base editing with minimal off-target effects in mammalian embryos

21 Oct 2025

Abstract excerpt

Developing glycosylase-based base editors (gBEs) to broaden the editing scope is highly desirable for biomedical research and agricultural applications. However, the off-target effects and applicability of gBEs need further investigation. We employ GOTI to detect rare DNA off-target events in mouse embryos injected with N-methylpurine glycosylase-based AYBE and gGBE. Transcriptome-wide RNA analysis reveals that...

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PMID: 41121388
DOI: 10.1186/s13059-025-03838-6

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