Article
Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice.
Nature communications - 30 Mar 2023
Yan Nana, Feng Hu, Sun Yongsen, Xin Ying, Zhang Haihang, Lu Hongjiang, Zheng Jitan, He Chenfei, Zuo Zhenrui, Yuan Tanglong, Li Nana, Xie Long, Wei Wu, Sun Yidi, Zuo Erwei
Abstract excerpt
Base editors have been reported to induce off-target mutations in cultured cells, mouse embryos and rice, but their long-term effects in vivo remain unknown. Here, we develop a Systematic evaluation Approach For gene Editing tools by Transgenic mIce (SAFETI), and evaluate the off-target effects of BE3, high fidelity version of CBE (YE1-BE3-FNLS) and ABE (ABE7.10F148A) in ~400 transgenic mice over 15 months....
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