Article
Artemis: Mass Spectrometry-Based Identification of MHC-Presented Peptides Across Alleles, Classes, and Species Using Soluble Single-Chain MHC Constructs.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026
Finton Kathryn A K, Foote-McNabb Ulysses N, Wilcox Elise C, Jones Lisa A, Gafken Philip R, Strong Roland K
Abstract excerpt
The current "gold standard" for the identification of MHC-restricted peptides is conventional immunoprecipitation/mass spectrometry (MS); however, this approach requires a relatively large amount of sample, complex purification procedures, and computational analyses to assign peptides to individual alleles. Here, we provide instructions for the expression, purification, and MS identification of MHC-presented...
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