Article
High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange.
Nature communications - 20 Apr 2020
Overall Sarah A, Toor Jugmohit S, Hao Stephanie, Yarmarkovich Mark, Sara M O'Rourke, Morozov Giora I, Nguyen Son, Japp Alberto Sada, Gonzalez Nicolas, Moschidi Danai, Betts Michael R, Maris John M, Smibert Peter, Sgourakis Nikolaos G
Abstract excerpt
Peptide exchange technologies are essential for the generation of pMHC-multimer libraries used to probe diverse, polyclonal TCR repertoires in various settings. Here, using the molecular chaperone TAPBPR, we develop a robust method for the capture of stable, empty MHC-I molecules comprising murine H2 and human HLA alleles, which can be readily tetramerized and loaded with peptides of choice in a high-throughput...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.