Article
RNA inosine sensor-guided TadA mutational scanning for toxicity minimization of adenine base editors.
Molecular therapy : the journal of the American Society of Gene Therapy - 7 Jan 2026
Zhang Cheng, Chen Ziliang, Cao Jixin, Zhang Zui, Li Wei-Ke, Zhang Xinyi, Chen Junying, Liu Jun, Yuan Zhou, Gao Feng, Shi Zehao, Zhao Xing-Ming, Chen Jingqi, Zhan Changyou, Cheng Tian-Lin
Abstract excerpt
The TadA component of adenine base editors (ABEs) induces widespread RNA off-target edits and raises safety concerns for their applications. However, the extent of RNA editing-related toxicity remains elusive, and high-throughput engineering of ABEs focusing on RNA editing activities remains challenging. Here, we demonstrate that RNA off-target editing of classical ABEs leads to substantial toxicity in vitro and...
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