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Article

Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau.

FEBS letters - 1 Oct 2025

Santambrogio Alessia, Lövestam Sofia, Metrick Michael A, Löhr Thomas, Xu Peifeng, Gallagher Nicholas C T, Ghetti Bernardino, Caughey Byron, Scheres Sjors H W, Vendruscolo Michele

Abstract excerpt

The assembly of tau into amyloid filaments is a hallmark of Alzheimer's disease (AD) and other tauopathies. Cryo-EM revealed the existence of disease-specific tau folds, which are challenging to replicate in vitro. We studied three full-length recombinant 0N3R tau forms (the wild-type and the C322A and C322S variants) using an RT-QuIC assay with brain homogenate seeding. C322A tau formed filaments resembling AD...

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Identifiers

PMID: 41026859
DOI: 10.1002/1873-3468.70141
PMCID: PMC12519061

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