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Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator

2 Feb 2023

Abstract excerpt

Yeast surface display is a valuable tool for protein engineering and directed evolution; however, significant variability in the copy number (i.e., avidity) of displayed variants on the yeast cell wall complicates screening and selection campaigns. Here, we report an engineered titratable display platform that modulates the avidity of Aga2-fusion proteins on the yeast cell wall dependent on the concentration of...

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PMID: 36728831
DOI: 10.1021/acssynbio.2c00351

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