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Article

Engineering the substrate specificity of the DhbE adenylation domain by yeast cell surface display.

Chemistry & biology - 24 Jan 2013

Zhang Keya, Nelson Kathryn M, Bhuripanyo Karan, Grimes Kimberly D, Zhao Bo, Aldrich Courtney C, Yin Jun

Abstract excerpt

The adenylation (A) domains of nonribosomal peptide synthetases (NRPSs) activate aryl acids or amino acids to launch their transfer through the NRPS assembly line for the biosynthesis of many medicinally important natural products. In order to expand the substrate pool of NRPSs, we developed a method based on yeast cell surface display to engineer the substrate specificities of the A-domains. We acquired A-domain...

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Identifiers

PMID: 23352143
DOI: 10.1016/j.chembiol.2012.10.020
PMCID: PMC3807743

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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