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Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

26 Feb 2018

Abstract excerpt

Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering an engineered protein to the cell wall by genetic fusion to one subunit of a dimeric yeast-mating agglutination receptor complex. This method enables an...

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PMID: 29482656
DOI: 10.1186/s12934-018-0881-3

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