Article
Single nucleotide polymorphism screening with denaturing gradient gel electrophoresis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Knapp Leslie A
Abstract excerpt
Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for identifying DNA sequence-based differences. The method relies on the fact that double-stranded DNA molecules have unique denaturation rates that are based upon the specific nucleotide composition of the DNA sequence(s). While DGGE is typically used to screen for polymorphisms that vary by multiple nucleotides, it is equally useful for...
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