Article
DFold: PCR design that minimizes secondary structure and optimizes downstream genotyping applications.
Human mutation - 1 Jul 2004
Fredman David, Jobs M, Strömqvist L, Brookes A J
Abstract excerpt
Secondary structures in polymerase chain reaction (PCR) target sequences have a negative impact on amplification success rates and on downstream uses of PCR products. For example, signal strength and allele discrimination in single nucleotide polymorphism (SNP) genotyping methods can be compromised by allele-biased amplification and/or by PCR product folding that limits access of interrogating probes. To increase...
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