Article
Special features of RAD Sequencing data: implications for genotyping.
Molecular ecology - 1 Jun 2013
Davey John W, Cezard Timothée, Fuentes-Utrilla Pablo, Eland Cathlene, Gharbi Karim, Blaxter Mark L
Abstract excerpt
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop or genotype markers accurately. We show that there are several sources of bias specific to RAD-Seq that are not explicitly addressed by current genotyping tools,...
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