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Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction.

Analytical biochemistry - 15 Apr 2005

Wu Wei-Ming, Tsai Hsiang-Ju, Pang Jong-Hwei S, Wang Hsin-Shih, Hong Hong-Shang, Lee Yun-Shien

Abstract excerpt

Different methods have been developed for single nucleotide polymorphism (SNP) typing during recent years. Allele-specific polymerase chain reaction (ASPCR) is a cost-saving method that scores SNPs by difference of the PCR efficiency of allele-specific primers. However, ASPCR for SNP typing is notoriously confounded for its locus-specific unpredictability and the laborious gel electrophoresis. In the current...

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PMID: 15797570
DOI: 10.1016/j.ab.2005.01.037

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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