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Double Digest Restriction-Site Associated DNA Sequencing (ddRAD-Seq).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Magbanua Zenaida V, Hsu Chuan-Yu, Pechanova Olga, Arick Mark, Peterson Daniel G

Abstract excerpt

ddRAD-Seq is a reduced representation sequencing technique that results in sequence datasets that can be compared and used to identify SNPs. We present an improved ddRAD-Seq protocol that increases efficiency and decreases the time to complete a ddRAD-Seq experiment. It utilizes selected restriction enzyme digestion fragments, quick acting ligases that are neutral with the restriction enzyme buffer eliminating...

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PMID: 40580296
DOI: 10.1007/978-1-0716-4642-7_16

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