Colorimetric loop-mediated isothermal amplification (cLAMP) assay for the genotyping of a thrombophilia genetic risk factor, MTHFR (C677T).

doi:10.1039/d5ay00215j

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Colorimetric loop-mediated isothermal amplification (cLAMP) assay for the genotyping of a thrombophilia genetic risk factor, MTHFR (C677T).

Analytical methods : advancing methods and applications - 29 May 2025

Altwayan Reham, Tombuloglu Huseyin, Alsaeed Moneerah, Alhusil Abdulrahman

Abstract excerpt

Methylenetetrahydrofolate reductase (MTHFR) is an enzyme involved in regulating serum homocysteine and folate levels. A single nucleotide polymorphism (SNP) of MTHFR (rs1801133; C677T) is strongly associated with an increased risk of thrombophilia, heart attack, stroke, and dementia. Genotyping this SNP is vital; however, techniques need to be simplified. This study developed a colorimetric loop-mediated...

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PMID: 40392249
DOI: 10.1039/d5ay00215j

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MTHFR

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