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Article

Development of an optimized RPA-PfAgo detection system for MTHFR C677T polymorphism genotyping.

Gene - 5 Sept 2024

Chen Lianghui, Wei Cheng, Liu Yaqun, Liao Meihua, Wang Jialin, Chen Jiaqi, Yang Peikui, Li Liejun, Xie Chengsong, Lin Min, Zhang Zhenxia, Zheng Yuzhong

Abstract excerpt

This study introduces an efficient RPA-PfAgo detection system for the MTHFR C677T polymorphism, proposing a potential strategy to simplify the genotyping process. By optimizing recombinase polymerase amplification (RPA) with Pyrococcus furiosus Argonaute (PfAgo) nucleases, we achieved DNA amplification at a constant temperature. The assay was fine-tuned through meticulous primer and guide DNA selection, with...

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Identifiers

PMID: 38734187
DOI: 10.1016/j.gene.2024.148544

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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