Article
Nanopore-based consensus sequencing enables accurate multimodal tumor cell-free DNA profiling.
Genome research - 14 Apr 2025
Chen Li-Ting, Jager Myrthe, Rebergen Dàmi, Brink Geertruid J, van den Ende Tom, Vanderlinden Willem, Kolbeck Pauline, Pagès-Gallego Marc, van der Pol Ymke, Besselink Nicolle, Moldovan Norbert, Hami Nizar, Kloosterman Wigard P, van Laarhoven Hanneke, Mouliere Florent, Zweemer Ronald, Lipfert Jan, Derks Sarah, Marcozzi Alessio, de Ridder Jeroen
Abstract excerpt
Shallow genome-wide cell-free DNA sequencing holds great promise for noninvasive cancer monitoring by providing reliable copy number alteration (CNA) and fragmentomic profiles. Single-nucleotide variations (SNVs) are, however, much harder to identify with low sequencing depth due to sequencing errors. Here, we present Nanopore Rolling Circle Amplification (RCA)-enhanced Consensus Sequencing (NanoRCS), which...
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