Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for Rapid, Accurate, and Affordable Somatic Mutation Detection

Abstract excerpt

Nanopore sequencing is more than 10-fold faster than sequencing-by-synthesis and provides reads that are roughly 100-fold longer. However, nanopore sequencing’s 7.5% intrinsic error rate renders it difficult to call somatic mutations with low variant allele frequencies (VAFs) without significant false positives. Here, we introduce the Oncogene Concatenated Enriched Amplicon Nanopore Sequencing (OCEANS) method, in...

Topics

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• Carcinoma, Non-Small-Cell Lung
• High-Throughput Nucleotide Sequencing
• Leukemia, Myeloid, Acute
• Lung Neoplasms
• Melanoma
• Nanopore Sequencing
• Neoplasms
• Oncogenes

Identifiers and source

Literature Corpus work

017ef019-879c-53ad-b3cc-f684f758ec1e

DOI

10.1101/2020.11.12.20230169