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Article

Protein engineering enables Serratia marcescens nuclease A to hydrolyze nucleic acids under high-salt conditions.

International journal of biological macromolecules - 1 Dec 2024

Qiu Cunjia, Chen Yaoxi, Wu Ruirui, Zhang Yifeng, Lang Meng, Gao Mengqiu, Cao Jian, Zhang Yujun, Chen Xiaoyue, Liao Shanhui

Abstract excerpt

For the purpose of removing nucleotide impurities, Serratia marcescens Nuclease A (SmNucA) is widespread used in biopharmaceutical manufacturing, such as production of adeno-associated viral vector, one of the leading gene delivery platforms that features low immunogenicity. However, such utilization of wild-type SmNucA is limited in saline environment. Depending on downstream process development, the ionic...

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PMID: 39566763
DOI: 10.1016/j.ijbiomac.2024.137860

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