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Article

Engineering Nucleotide Specificity of Succinyl-CoA Synthetase in Blastocystis: The Emerging Role of Gatekeeper Residues.

Biochemistry - 24 Jan 2017

Vashisht Kapil, Verma Sonia, Gupta Sunita, Lynn Andrew M, Dixit Rajnikant, Mishra Neelima, Valecha Neena, Hamblin Karleigh A, Maytum Robin, Pandey Kailash C, van der Giezen Mark

Abstract excerpt

Charged, solvent-exposed residues at the entrance to the substrate binding site (gatekeeper residues) produce electrostatic dipole interactions with approaching substrates, and control their access by a novel mechanism called "electrostatic gatekeeper effect". This proof-of-concept study demonstrates that the nucleotide specificity can be engineered by altering the electrostatic properties of the gatekeeper...

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Identifiers

PMID: 27478903
DOI: 10.1021/acs.biochem.6b00098
PMCID: PMC5404824

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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