Article
Probing the mechanism of staphylococcal nuclease with unnatural amino acids: kinetic and structural studies.
Science (New York, N.Y.) - 17 Sept 1993
Judice J K, Gamble T R, Murphy E C, de Vos A M, Schultz P G
Abstract excerpt
Staphylococcal nuclease is an enzyme with enormous catalytic power, accelerating phosphodiester bond hydrolysis by a factor of 10(16) over the spontaneous rate. The mechanistic basis for this rate acceleration was investigated by substitution of the active site residues Glu43, Arg35, and Arg87 wi...
Topics
- 2-Aminoadipic Acid
- Amino Acids
- Aminobutyrates
- Arginine
- Binding Sites
- Catalysis
- Glutamates
- Glutamic Acid
- Homocysteine
- Hydrogen Bonding
- Hydrogen-Ion Concentration
- Kinetics
- Micrococcal Nuclease
- Mutation
- Plasmids
- X-Ray Diffraction