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Exploration of urease-aided calcium carbonate mineralization by enzyme analyses of Neobacillus mesonae strain NS-6.

Microbiology spectrum - 7 Jan 2025

Ma Zhiwei, Chen Mengyao, Lu Juncheng, Liu Shichuang, Ma Yanling

Abstract excerpt

Urease containing nickel cofactor is crucial for urea-hydrolytic induced calcium carbonate (CaCO3) precipitation (UICP). However, limited information exists regarding the influence of amino acid residues interacting with nickel ions in its structure on induced CaCO3 mineralization. Herein, RT-qPCR was used to demonstrate that the addition of NiCl2 dramatically upregulated the expression of urease structural gene...

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PMID: 39611830
DOI: 10.1128/spectrum.01891-24

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