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Article

Engineering the residues on "A" surface and C-terminal region to improve thermostability of nitrilase.

Enzyme and microbial technology - 1 Jun 2018

Xu Zhe, Cai Ting, Xiong Neng, Zou Shu-Ping, Xue Ya-Ping, Zheng Yu-Guo

Abstract excerpt

Nitrilases can hydrolyze nitriles to corresponding carboxylic acids in one single step, which have great potential as valuable biocatalysts for chemical synthesis. However, the poor thermostability of the nitrilases restrict their applications in industry. In this work, error-prone PCR and site-directed mutagenesis were utilized to improve the thermostability of nitrilases. Several mutants (AcN-Q339K, AcN-Q343K,...

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Identifiers

PMID: 29602387
DOI: 10.1016/j.enzmictec.2018.03.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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