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Article

Enhancement of nucleoside phosphorylation activity in an acid phosphatase.

Protein engineering - 1 Jul 2002

Ishikawa Kohki, Mihara Yasuhiro, Shimba Nobuhisa, Ohtsu Naoko, Kawasaki Hisashi, Suzuki Ei-ichiro, Asano Yasuhisa

Abstract excerpt

Escherichia blattae non-specific acid phosphatase (EB-NSAP) possesses a pyrophosphate-nucleoside phosphotransferase activity, which is C-5'-position selective. Current mutational and structural data were used to generate a mutant EB-NSAP for a potential industrial application as an effective and economical protein catalyst in synthesizing nucleotides from nucleosides. First, Gly74 and Ile153 were replaced by Asp...

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Source

Identifiers

PMID: 12200535
DOI: 10.1093/protein/15.7.539

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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