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Article

Performance of an in-house multiplex PCR assay for HIV-1 drug resistance testing - A cheaper alternative.

Journal of virological methods - 1 Dec 2024

Fortuin Tumelo L, Nkone Paballo, Glass Allison, Viana Raquel, Moeng Keitumetse, Loubser Shayne, Tiemessen Caroline T, Mayaphi Simnikiwe H

Abstract excerpt

BACKGROUND: Currently, most HIV drug resistance PCR assays amplify the protease-reverse transcriptase (PR-RT) fragment separately from the integrase (IN) fragment. The aim of this study was to develop a multiplex PCR assay that simultaneously amplifies PR-RT and IN fragments for HIV-1 drug-resistance testing. METHODS: The in-house multiplex PCR assay was evaluated on extracted total nucleic acids obtained from...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 39303923
DOI: 10.1016/j.jviromet.2024.115034

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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