Article
A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance.
Journal of virological methods - 1 May 2006
Steegen Kim, Demecheleer Els, De Cabooter Nancy, Nges Dieudonné, Temmerman Marleen, Ndumbe Peter, Mandaliya Kishor, Plum Jean, Verhofstede Chris
Abstract excerpt
Quantification of the viral burden and identification of drug resistant mutations are important laboratory tools in the management of HIV-1 infected patients. However, widespread use of assays for viral load determination and genotyping is still hampered by the high cost. Here, an in-house RT-PCR...
Topics
- Anti-HIV Agents
- Base Sequence
- Cohort Studies
- Costs and Cost Analysis
- Drug Resistance, Viral
- Female
- Genes, Viral
- Genotype
- HIV Infections
- HIV Protease
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Mutation
- Nucleic Acid Amplification Techniques