Investigation of drug resistance against protease, reverse transcriptase, and integrase inhibitors by next-generation sequencing in HIV-positive patients.

Abstract excerpt

Our aim was to investigate the mutations in protease (PR), reverse transcriptase (RT), and integrase (IN) gene regions in human immunodeficiency virus (HIV) using a single amplicon via next-generation sequencing (NGS). The study included plasma samples from 49 HIV-1-positive patients, which were referred for HIV-1 drug resistance testing during 2017. A nested polymerase chain reaction (PCR) was performed after...

Topics

• Anti-HIV Agents
• Drug Resistance, Viral
• Genome, Viral
• Genotype
• HIV Infections
• HIV Protease Inhibitors
• HIV-1
• High-Throughput Nucleotide Sequencing
• Humans
• Integrase Inhibitors