Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization of a low cost and broadly sensitive genotyping assay for HIV-1 drug resistance surveillance and monitoring in resource-limited settings.

PloS one - 1 Jan 2011

Zhou Zhiyong, Wagar Nick, DeVos Joshua R, Rottinghaus Erin, Diallo Karidia, Nguyen Duc B, Bassey Orji, Ugbena Richard, Wadonda-Kabondo Nellie, McConnell Michelle S, Zulu Isaac, Chilima Benson, Nkengasong John, Yang Chunfu

Abstract excerpt

UNLABELLED: Commercially available HIV-1 drug resistance (HIVDR) genotyping assays are expensive and have limitations in detecting non-B subtypes and circulating recombinant forms that are co-circulating in resource-limited settings (RLS). This study aimed to optimize a low cost and broadly sensitive in-house assay in detecting HIVDR mutations in the protease (PR) and reverse transcriptase (RT) regions of pol...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Biological Assay

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Costs and Cost Analysis

Share to Developing Countries

Share to Dried Blood Spot Testing

Share to Genotyping Techniques

Share to Indicators and Reagents

Share to Population Surveillance

Source

Open in PubMed Open full text

Identifiers

PMID: 22132237
DOI: 10.1371/journal.pone.0028184
PMCID: PMC3223235

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization of a low cost and broadly sensitive genotyping assay for HIV-1 drug resistance surveillance and monitoring in resource-limited settings.PMID 22132237Read ↗

Field evaluation of a broadly sensitive HIV-1 in-house genotyping assay for use with both plasma and dried blood spot specimens in a resource-limited country.Semantic proximity Read ↗

Evaluation of a cost effective in-house method for HIV-1 drug resistance genotyping using plasma samples.Semantic proximity Read ↗

Affordable in-house antiretroviral drug resistance assay with good performance in non-subtype B HIV-1.Semantic proximity Read ↗

Performance characteristics of a modified HIV-1 drug resistance genotyping method for use in resource-limited settingsSemantic proximity Read ↗

Development and application of a broadly sensitive dried-blood-spot-based genotyping assay for global surveillance of HIV-1 drug resistance.Semantic proximity Read ↗

Evaluation of an in-house genotyping resistance test for HIV-1 drug resistance interpretation and genotyping.Semantic proximity Read ↗

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited SettingsSemantic proximity Read ↗

A pragmatic approach to HIV-1 drug resistance determination in resource-limited settings by use of a novel genotyping assay targeting the reverse transcriptase-encoding region only.Semantic proximity Read ↗

HIV-1 Drug Resistance Genotyping in Resource Limited Settings: Current and Future Perspectives in Sequencing Technologies.Semantic proximity Read ↗

A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens.Semantic proximity Read ↗

Dried Blood Spot Specimens Are a Suitable Alternative Sample Type for HIV-1 Viral Load Measurement and Drug Resistance Genotyping in Patients Receiving First-Line Antiretroviral TherapySemantic proximity Read ↗

Affordable drug resistance genotyping of HIV-1 reverse transcriptase, protease and integrase genes, for resource limited settings.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.