Article
Discrete measurements of RNA polymerase and reverse transcriptase fidelity reveal evolutionary tuning.
RNA (New York, N.Y.) - 16 Aug 2024
Potapov Vladimir, Krudup Stanislas, Maguire Sean, Unlu Irem, Guan Shengxi, Buss Jackson A, Smail Benedict A, van Eeuwen Trevor, Taylor Martin S, Burns Kathleen H, Ong Jennifer L, Trachman Robert J
Abstract excerpt
Direct methods for determining the fidelity of DNA polymerases are robust, with relatively little sample manipulation before sequencing. In contrast, methods for measuring RNA polymerase and reverse transcriptase fidelities are complicated by additional preparation steps that introduce ambiguity and error. Here, we describe a sequencing method, termed Roll-Seq, for simultaneously determining the individual...
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