A high-throughput assay for quantitative measurement of PCR errors.

doi:10.1038/s41598-017-02727-8

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Article

A high-throughput assay for quantitative measurement of PCR errors.

Scientific reports - 2 Jun 2017

Shagin Dmitriy A, Shagina Irina A, Zaretsky Andrew R, Barsova Ekaterina V, Kelmanson Ilya V, Lukyanov Sergey, Chudakov Dmitriy M, Shugay Mikhail

Abstract excerpt

The accuracy with which DNA polymerase can replicate a template DNA sequence is an extremely important property that can vary by an order of magnitude from one enzyme to another. The rate of nucleotide misincorporation is shaped by multiple factors, including PCR conditions and proofreading capabilities, and proper assessment of polymerase error rate is essential for a wide range of sensitive PCR-based assays. In...

Topics

Alleles
Analysis of Variance
Gene Library
High-Throughput Nucleotide Sequencing
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Sequence Analysis, DNA

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Identifiers

PMID: 28578414
DOI: 10.1038/s41598-017-02727-8
PMCID: PMC5457411

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