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Article

TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.

Nature communications - 29 Nov 2016

Picher Ángel J, Budeus Bettina, Wafzig Oliver, Krüger Carola, García-Gómez Sara, Martínez-Jiménez María I, Díaz-Talavera Alberto, Weber Daniela, Blanco Luis, Schneider Armin

Abstract excerpt

Sequencing of a single-cell genome requires DNA amplification, a process prone to introducing bias and errors into the amplified genome. Here we introduce a novel multiple displacement amplification (MDA) method based on the unique DNA primase features of Thermus thermophilus (Tth) PrimPol. TthPrimPol displays a potent primase activity preferring dNTPs as substrates unlike conventional primases. A combination of...

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PMID: 27897270
DOI: 10.1038/ncomms13296
PMCID: PMC5141293

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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