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Article

Partial bisulfite conversion for unique template sequencing.

Nucleic acids research - 25 Jan 2018

Kumar Vijay, Rosenbaum Julie, Wang Zihua, Forcier Talitha, Ronemus Michael, Wigler Michael, Levy Dan

Abstract excerpt

We introduce a new protocol, mutational sequencing or muSeq, which uses sodium bisulfite to randomly deaminate unmethylated cytosines at a fixed and tunable rate. The muSeq protocol marks each initial template molecule with a unique mutation signature that is present in every copy of the template, and in every fragmented copy of a copy. In the sequenced read data, this signature is observed as a unique pattern of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29161423
DOI: 10.1093/nar/gkx1054
PMCID: PMC5778454

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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