Article

Dissecting and Tuning Primer Editing by Proofreading Polymerases

2021-05-12

Abstract excerpt

<h4>ABSTRACT</h4> Proofreading polymerases have 3’ to 5’ exonuclease activity that allows the excision and correction of mis-incorporated bases during DNA replication. In a previous study, we demonstrated that in addition to correcting substitution errors and lowering the error rate of DNA amplification, proofreading polymerases can also edit PCR primers to match template sequences. Primer editing is a feature th...

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Literature Corpus work: de722339-066c-583c-9fc8-02bc333dc5a5
DOI: 10.1101/2021.05.11.443694

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