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Article

Library preparation for highly accurate population sequencing of RNA viruses.

Nature protocols - 1 Jul 2014

Acevedo Ashley, Andino Raul

Abstract excerpt

Circular resequencing (CirSeq) is a novel technique for efficient and highly accurate next-generation sequencing (NGS) of RNA virus populations. The foundation of this approach is the circularization of fragmented viral RNAs, which are then redundantly encoded into tandem repeats by 'rolling-circle' reverse transcription. When sequenced, the redundant copies within each read are aligned to derive a consensus...

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Identifiers

PMID: 24967624
DOI: 10.1038/nprot.2014.118
PMCID: PMC4418788

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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