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Article

[DNA assembly by multi-fragment digestion/ligation and homologous recombination].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 May 2024

Rao Xinyue, Chong Jinyang, He Cheng, Bi Yingying, Tang Gangmin, Lü Yucai, Gong Dachun, Yang Xiao

Abstract excerpt

To develop an accurate and efficient protocol for multi-fragment assembly and multi-site mutagenesis, we integrated and optimized the common multi-fragment assembly methods and validated the established method by using fructose-1,6-diphosphatase 1 (FBP1) with 4 mutant sites. The fragments containing mutations were assembled by introducing mutant sites and Bsa I recognition sequences. After digestion/ligation, the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 38783816
DOI: 10.13345/j.cjb.230793

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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