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Article

A convenient and robust method for construction of combinatorial and random mutant libraries.

Biochimie - 1 Aug 2010

An Yingfeng, Wu Wenfang, Lv Anguo

Abstract excerpt

Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The product is then mixed with linearized vector and two pairs of flanking primers, followed by...

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PMID: 20350580
DOI: 10.1016/j.biochi.2010.03.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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