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[Using multiple-fragment amplification combined with Gibson assembly to clone genes with site-directed mutations].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 Mar 2022

Cheng Yingying, Li Guoqing, Liu Junyi, Chen Wanyu, Chen Huabo

Abstract excerpt

In order to develop a simple and efficient site-directed mutagenesis solution, the Gibson assembly technique was used to clone the cyclin dependent kinase 4 gene with single or double site mutations, with the aim to simplify the overlap extension PCR. The gene fragments containing site mutations were amplified using a strategy similar to overlap extension PCR. Meanwhile, an empty plasmid was digested by double...

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Identifiers

PMID: 35355487
DOI: 10.13345/j.cjb.210914

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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