Article
In-Fusion BioBrick assembly and re-engineering.
Nucleic acids research - 1 May 2010
Sleight Sean C, Bartley Bryan A, Lieviant Jane A, Sauro Herbert M
Abstract excerpt
Genetic circuits can be assembled from standardized biological parts called BioBricks. Examples of BioBricks include promoters, ribosome-binding sites, coding sequences and transcriptional terminators. Standard BioBrick assembly normally involves restriction enzyme digestion and ligation of two BioBricks at a time. The method described here is an alternative assembly strategy that allows for two or more...
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