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Article

Multiplex site-directed mutagenesis strategy including high-efficiency selection of the mutant PCR products.

Biotechnology letters - 1 Dec 2007

Varga-Orvos Zoltán, Nagy Zsolt B, Mészáros Attila, Kökény Szabolcs, Gergely Péter, Tamás László, Poór Gyula

Abstract excerpt

Site-directed mutagenesis is of great importance for probing the structure/function relationship of proteins. Developing our previous method (Nagy et al. Anal Biochem 324:301-303, 2004), here we report a multiplex strategy for site-directed mutagenesis using PCR in one tube to introduce a single mutation into three or more genes at the same time. DNA fragments carrying the desired mutation can be distinguished...

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Share to Cloning, Molecular
Share to DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Restriction Enzymes

Share to Genetic Vectors

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Polymerase Chain Reaction

Share to Selection, Genetic

Source

Identifiers

PMID: 17687622
DOI: 10.1007/s10529-007-9474-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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