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Article

An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.

ACS synthetic biology - 19 Apr 2024

Lindeboom Timon A, Sanchez Olmos María Del Carmen, Schulz Karina, Brinkmann Cedric K, Ramírez Rojas Adán A, Hochrein Lena, Schindler Daniel

Abstract excerpt

Synthetic Sc2.0 yeast strains contain hundreds to thousands of loxPsym recombination sites that allow restructuring of the Saccharomyces cerevisiae genome by SCRaMbLE. Thus, a highly diverse yeast population can arise from a single genotype. The selection of genetically diverse candidates with rearranged synthetic chromosomes for downstream analysis requires an efficient and straightforward workflow. Here we...

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PMID: 38597458
DOI: 10.1021/acssynbio.3c00476
PMCID: PMC11036488

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Source receipt: pubmed, retrieved 7 Sept 2026.

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