Evaluating the mutagenicity of N-nitrosodimethylamine in 2D and 3D HepaRG cell cultures using error-corrected next generation sequencing.

doi:10.1007/s00204-024-03731-4

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Article

Evaluating the mutagenicity of N-nitrosodimethylamine in 2D and 3D HepaRG cell cultures using error-corrected next generation sequencing.

Archives of toxicology - 1 Jun 2024

Seo Ji-Eun, Le Yuan, Revollo Javier, Miranda-Colon Jaime, Xu Hannah, McKinzie Page, Mei Nan, Chen Tao, Heflich Robert H, Zhou Tong, Robison Timothy, Bonzo Jessica A, Guo Xiaoqing

Abstract excerpt

Human liver-derived metabolically competent HepaRG cells have been successfully employed in both two-dimensional (2D) and 3D spheroid formats for performing the comet assay and micronucleus (MN) assay. In the present study, we have investigated expanding the genotoxicity endpoints evaluated in He...

Topics

Humans
Dimethylnitrosamine
High-Throughput Nucleotide Sequencing
Comet Assay
Micronucleus Tests
Mutagens
DNA Damage
Spheroids, Cellular
Mutagenicity Tests
Cell Culture Techniques
Cell Line
Hepatocytes
Mutagenesis

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Identifiers

PMID: 38584193
DOI: 10.1007/s00204-024-03731-4
PMCID: PMC11106104

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-38584193-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.