Article
Genetic toxicity testing using human in vitro organotypic airway cultures: Assessing DNA damage with the CometChip and mutagenesis by Duplex Sequencing.
Environmental and molecular mutagenesis - 1 Jun 2021
Wang Yiying, Mittelstaedt Roberta A, Wynne Rebecca, Chen Ying, Cao Xuefei, Muskhelishvili Levan, Davis Kelly, Robison Timothy W, Sun Wei, Schmidt Elizabeth K, Smith Thomas H, Norgaard Zachary K, Valentine Charles C, Yaplee Jeffry, Williams Lindsey N, Salk Jesse J, Heflich Robert H
Abstract excerpt
The organotypic human air-liquid-interface (ALI) airway tissue model has been used as an in vitro cell culture system for evaluating the toxicity of inhaled substances. ALI airway cultures are highly differentiated, which has made it challenging to evaluate genetic toxicology endpoints. In the current study, we assayed DNA damage with the high-throughput CometChip assay and quantified mutagenesis with Duplex...
Topics
- DNA Damage
- Epithelial Cells
- Ethyl Methanesulfonate
- Humans
- Mutagenesis
- Mutagenicity Tests
- Mutagens
- Mutation
- Respiratory System