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Article

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9.

Nature communications - 22 Dec 2020

Álvarez Beatriz, Mencía Mario, de Lorenzo Víctor, Fernández Luis Ángel

Abstract excerpt

In vivo mutagenesis systems accelerate directed protein evolution but often show restricted capabilities and deleterious off-site mutations on cells. To overcome these limitations, here we report an in vivo platform to diversify specific DNA segments based on protein fusions between various base deaminases (BD) and the T7 RNA polymerase (T7RNAP) that recognizes a cognate promoter oriented towards the target...

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Identifiers

PMID: 33353963
DOI: 10.1038/s41467-020-20230-z
PMCID: PMC7755918

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