Article
AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns.
Nucleic acids research - 20 May 2022
Liang Yanhui, Xie Jingke, Zhang Quanjun, Wang Xiaomin, Gou Shixue, Lin Lihui, Chen Tao, Ge Weikai, Zhuang Zhenpeng, Lian Meng, Chen Fangbing, Li Nan, Ouyang Zhen, Lai Chengdan, Liu Xiaoyi, Li Lei, Ye Yinghua, Wu Han, Wang Kepin, Lai Liangxue
Abstract excerpt
Establishing saturated mutagenesis in a specific gene through gene editing is an efficient approach for identifying the relationships between mutations and the corresponding phenotypes. CRISPR/Cas9-based sgRNA library screening often creates indel mutations with multiple nucleotides. Single base editors and dual deaminase-mediated base editors can achieve only one and two types of base substitutions,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.