Article
Targeted C-to-T and A-to-G dual mutagenesis system for RhtA transporter in vivo evolution.
Applied and environmental microbiology - 31 Oct 2023
Wei Zhandong, Zhao Dongdong, Wang Jie, Li Ju, Xu Ning, Ding Chao, Liu Jun, Li Siwei, Zhang Chunzhi, Bi Changhao, Zhang Xueli
Abstract excerpt
T7 RNA polymerase (T7RNAP) has been fused with cytosine or adenine deaminase individually, enabling in vivo C-to-T or A-to-G transitions on DNA sequence downstream of T7 promoter, and greatly accelerated directed protein evolution. However, its base conversion type is limited. In this study, we created a dual-functional system for simultaneous C-to-T and A-to-G in vivo mutagenesis, called T7-DualMuta, by fusing...
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