Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Broad spectrum of CRISPR-induced edits in an embryonic lethal gene.

Scientific reports - 9 Dec 2021

Fuselier Kayla T B, Salbaum J Michael, Kappen Claudia

Abstract excerpt

Mendelian genetics poses practical limitations on the number of mutant genes that can be investigated simultaneously for their roles in embryonic development in the mouse. While CRISPR-based gene editing of multiple genes at once offers an attractive alternative strategy, subsequent breeding or establishment of permanent mouse lines will rapidly segregate the different mutant loci again. Direct phenotypic...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Embryonic Development
Share to Gene Editing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Lethal

Share to Genetic Engineering

Share to INDEL Mutation

Share to Mutagenesis

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 34887431
DOI: 10.1038/s41598-021-02627-y
PMCID: PMC8660864

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Broad spectrum of CRISPR-induced edits in an embryonic lethal gene.PMID 34887431Read ↗

Analysing the outcome of CRISPR-aided genome editing in embryos: Screening, genotyping and quality control.Semantic proximity Read ↗

Universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporationSemantic proximity Read ↗

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome.Semantic proximity Read ↗

Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing.Semantic proximity Read ↗

Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system.Semantic proximity Read ↗

A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research.Semantic proximity Read ↗

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.Semantic proximity Read ↗

Evaluation of CRISPR gene-editing tools in zebrafishSemantic proximity Read ↗

The use of CRISPR/Cas9-based gene editing strategies to explore cancer gene function in mice.Semantic proximity Read ↗

Efficient strategies to detect genome editing and integrity in CRISPR-Cas9 engineered ESCsSemantic proximity Read ↗

One-step generation of phenotype-expressing triple-knockout mice with heritable mutated alleles by the CRISPR/Cas9 system.Semantic proximity Read ↗

Optimizing gRNA selection for high-penetrance F0 CRISPR screening for interrogating disease gene function.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.